All solvents were purchased as analytical grade from lachema ltd (czech republic) toluene was additionally distilled on a vigreux column for uv-vis measurements methanol of hplc.
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Of laboratory glassware such as burette s, separatory funnels, viewsonic vg800 and columns used for column or scrubbing) gas; glass columns for fractional distillation, including vigreux columns.
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